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| VOLUME 98 (2013) | ISSUE 11 | PAGE 830 |
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Low-frequency dynamics of DNA in Brillouin light scattering spectra1)
S. G. Lushnikov+*, A. V. Dmitriev+*, A. I. Fedoseev*, G. A. Zakharov*×, A. V. Zhuravlev×, A. V. Medvedeva*×, B. F. Schegolev×, E. V. Savvateeva-Popova*×
+Ioffe Physico-Technical Institute of the RAS, 194021 St. Petersburg, Russia *St. Petersburg State University, 199034 St. Petersburg, Russia ×Pavlov Institute of Physiology, 199034 St. Petersburg, Russia
Abstract
Brillouin light scattering studies of deoxyribonucleic acid (DNA) in the temperature interval 297-375 K are presented. DNA fragment (119 bp) from the 1st intron of D melanogaster limk1/ gene with AT-rich insertion (28 bp, mutant agnts3) was used as an experimental sample. The temperature dependence of the hypersound velocity was found to exhibit anomalies in the vicinity of 347 and 335 К. Computer modeling of possible conformational states which might be attained by the DNA fragment under study has shown the existence of local structures that evolve with varying temperature. Combined analysis of experimental data and results of the modeling reveals a close relation between the anomalous behavior of Brillouin light scattering spectra and conformational DNA dynamics. The results are discussed in the framework of modern models of conformational DNA transformations. |